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PRIMACYT Hepatocyte Maintenance Media

Advanced Cell Cultures Media – Long and Short Term Culturing



Benefit from PRIMACYTas European Reference Laboratory for Alternatives to Animal Testing.


PRIMACYT maintenance media are your choice for the in vitro cultivation and maintenance of primary hepatocytes. Our suite of cell culture media support the creation of your almost perfect hepatic model by stabilizing the expression of metabolism capacity and uptake / efflux transporters.

The PRIMACYT Short and Long term Cell Culture media are part of our serum free cell culture media program. Chemically defined – Animal Component Free or CLASSIC – All Serum Free.




PRIMACYT as a member of the European Reference Laboratory Network for Alternatives to Animal Testing. We strongly support the use of serumfree media for culturing cells.

PRIMACYT hepatocyte maintenance products contain all required growth and epidermal growth factors. Altogether, our long term media are adapted to allow cell culturing more than four weeks, depending on donor cells. Our studies show favorable detoxification and albumin synthesis results as well as inducible Cytochrome P450 protein expression and enzymatic activities.

Delivery
  • Basal Medium and Medium Ready-to-use at ambient temperature (500 ml or 250 ml bottle)
    • Supplements 1 and 2 in vials at ambient temperature
    • Supplements 3, 4 and 5 in vials on dry ice
Storage:
  • Basal Medium and Medium Ready-to-use at 4 deg C
  • Supplements 1 and 2 at 4 deg C
  • Supplement 3 at -20 deg C
  • Supplements 4 and 5 at -80 deg C
Our maintenance media help you in saving time and expenses, accelerating your R&D activities. We offer two different cell culture media types, optimized for short/long term culturing, all chemically defined and either animal component free or as classic media. To accomplish your needs and for your convenience, media are offered either ready to use with a shelf life of 1 month or, with an increased shelf life, as basal media plus supplements.


Features / Benefits

  • Perfect short term and long term cell culturing instrument to obtain higher cell viability at proper cell morphology.
  • Optimized for human hepatocytes
  • Suited also for dog (beagle) and monkey (cynomolgus) hepatocytes.
  • Restores and maintains cytochrome P450 and enzymatic activity over long time.
  • Maintains and supports efflux and uptake transporter activities
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Applications

  • Study of metabolic processes.
  • Drug metabolism and other cytology studies.
  • Pharmacology studies.
  • Hepatotoxicity studies.
  • Basic research and drug discovery studies.
  • Drug-drug interaction studies.
  • Analysis of transporter activities.
  • Employment in bioprocess assays for the development of vaccines, pharmaceuticals, diagnostics and therapeutics.
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Availability Long Term Maintenance Medium


ACF-HHMM-500S
Animal Component Free Human Hepatocyte Maintenance Medium, Basal medium with supplements (500 ml)

ACF-HHMM-500
Animal free Human Hepatocyte Maintenance Medium, Ready-to-use (500 ml)

ACF-HHMM-250
Animal free medium Human Hepatocyte Maintenance Medium, Ready-to-use (250 ml)

HHMM-500S
Human Hepatocyte Maintenance Medium, Basal medium with supplements (500 ml)

HHMM-500
Human Hepatocyte Maintenance Medium, Ready-to-use (500 ml)


HHMM-250
Human Hepatocyte Maintenance Medium, Ready-to-use (250 ml)

Availability Short Term Maintenance Medium


ACF-HM-500S
Animal Component Free Human Hepatocyte Maintenance Medium, Basal medium with supplements (500 ml)

ACF-HM-500
Animal free Human Hepatocyte Maintenance Medium, Ready-to-use (500 ml)

ACF-HM-250
Animal free medium Human Hepatocyte Maintenance Medium, Ready-to-use (250 ml)

HM-500S
Human Hepatocyte Maintenance Medium, Basal medium with supplements (500 ml)

HM-500
Human Hepatocyte Maintenance Medium, Ready-to-use (500 ml)

HM-250
Human Hepatocyte Maintenance Medium, Ready-to-use (250 ml)



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