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… Please note that minimum order volumes of 5-10L may apply for custom made SILAC media not commonly used in research laboratories except for DMEM, DMEM/F12 and RPMI 1640 which we normally have in stock.





    Dulbecco’s modified Eagle's medium (DMEM)

    • default_titleDulbecco modified Eagle's media - Dulbecco Modified, (DMEM), contain approximately four times as much of the vitamins and amino acids present in the original formula (modified Eagle's minimal essential medium, MEM) and two to four times as much glucose. Additionally, it contains iron and phenol red.
    • default_titleOur DMEM medium is optimized for use with our dialyzed fetal calf bovine and horse serum.
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    • default_titleDMEM is suitable for most types of cells, including PRIMACYT human, monkey, rat, mouse, and Goettingen minipig cells.
    • default_titleMedia are shipped at room temperature and should be stored at 4-8°C immediately after arrival.
    • default_titleDMEM is offered ready to use in bottles sterile packed at 500 ml each.
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    Short Form
    Order No.

    Media

    L-Arginine
    L-Lysine
    None
    DMEM-10-LM010
    SILAC DMEM Media without arginine and lysine amino acids
    No L-Arginine
    No Label
    No L-Lysine
    No Label
    R0K0
    DMEM-14-LM014
    Control SILAC DMEM media containing unlabelled arginine and lysine amino acids (R0K0)
    No Label
    No Label
    R6K6
    DMEM-11-LM011
    Ready to use SILAC DMEM media containing 13C labelled arginine and lysine amino acids (R6K6)
    13 C
    13 C
    R10K8
    DMEM-15-LM015
    Ready to use SILAC DMEM media containing 13C and 15N labelled arginine, and13C and 15N labelled lysine (R10K8). Normally used in triple labelling experiments
    13 C
    15 N
    13 C
    15 N
    R6K4
    DMEM-16-LM016
    Normally used in triple labeling experiments.
    Ready to use SILAC DMEM media containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)
    13 C
    2 D
    R6K8
    DMEM-17-LM017
    Normally used in triple labeling experiments.
    Ready-to-use SILAC DMEM Media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)
    13 C
    13 C
    15 N



    SILAC RPMI 1640 Media – Optimized for GrowthReady to Use – Convenience in Mass Spectrometry

    RPMI 1640 media are used in cell culture and tissue culture. RPMI 1640 uses a bicarbonate buffering system. The RPMI composition is optimized for amino acids and vitamins and typically shows a pH 8 value.

    The RPMI 1640, originally developed by the Roswell Park Memorial Institute, contains no growth factor or proteins. Therefore supplemental serum or an adequate replacement medium are required. RPMI is used for the cultivation of a large spectrum of cell types, including mammalian cells, hybrid cells and in hybridoma fusion protocols. Various components may be added apart from amino acids, vitamins, serum or serum replacements, such as growth factors and cytokines.

    SILAC - Stable Isotope Labeling with Amino Acids in Cell Culture – RPMI 1640 - is your choice for Mass Spectroscopy MS based quantitative proteomics.

    RPMI 1640 media are used in cell culture and tissue culture for growing cells, for example HeLa, MCF-7, PBMC and others. Key application areas are
    - Protein identification
    - Biomarker Discovery
    - Proteome Analysis
    - Determination of peptide turnover kinetics.

    RPMI 1640 Medium

    We offer several SILAC RPMI 1640 media with different label combinations for L-arginine and L-lysine, 13C, 15N and D2 (R6K4, R6K6, R6K8 and R10K8 respectively and control mwdium R0K0) as well as RPMI without arginine and lysine. Each product is bottled at a volume of 500ml. Products do not contain L-Glutamine and Sodium Pyruvate.

    The principle of SILAC.


    Cells are differentially labeled by growing them in light medium with normal arginine (Arg-0, blue color) or medium with heavy arginine (Arg-6, red color). Metabolic incorporation of the amino acids into the proteins results in a mass shift of the corresponding peptides. This mass shift can be detected by a mass spectrometer as indicated by the depicted mass spectra. When both samples are combined, the ratio of peak intensities in the mass spectrum reflects the relative protein abundance. In this example, the labeled protein has the same abundance in both samples (ratio 1).

    Click graph to enlarge.

    How SILAC works
    Text and graphics under public license from wikipedia.org

    Order Number
    Media Description

    Packaging, Bottle

    RPMI-18-LM018
    Ready-to-use SILAC RPMI 1640 Media containing 13C labelled arginine and lysine amino acids (R6K6). w/o L-Glutamine, w/o Sodium Pyruvate

    500 ml

    RPMI-19-LM019
    Control SILAC RPMI 1640 Media containing unlabelled arginine and lysine amino acids (R0K0). w/o L-Glutamine, w/o Sodium Pyruvate

    500 ml

    RPMI-20-LM020
    Ready-to-use SILAC RPMI 1640 Media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8). w/o L-Glutamine, w/o Sodium Pyruvate

    500 ml

    RPMI-21-LM021
    Normally used in triple labeling experiments.
    Ready-to-use SILAC RPMI 1640 Media containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8). w/o L-Glutamine, w/o Sodium Pyruvate

    500 ml

    RPMI-22-LM022
    Normally used in triple labeling experiments.
    Ready to use SILAC RPMI 1640 media containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4). w/o L-Glutamine, w/o Sodium Pyruvate

    500 ml



    Notes: You can retrieve this product under the terms rpmi 1640 media, rpmi, rpmi 1640, rpmi media, rpmi medium and rpmi 1640 medium.



    Leibovitz L-15 MediumDevelop – Support – Grow - Proliferate

    SILAC Stable isotope labeling by amino acids in cell culture is a well known technique for detection of differences in protein abundance among samples using non-radioactive isotopic labeling and mass spectrometry.

    Leibovitz’s L15 media enhance and prolongate the proliferation of cells in a CO2 free environment. The L-15 Medium (Leibovitz) has been formulated without sodium bicarbonate buffer systems for cells growing in a carbon dioxide ( CO2 ) free atmosphere. The Leibovitz’s L-a5 medium is buffered by its own salt, galactose and free base amino acids. As a result, L-15 media support a better physiological control of its pH value.
    SILAC Leibovitz’s L-15 media incorporate L-Arginine and L-Lysine isotopes for quantitative proteomics. We offer following isotopes for the comparison of two (duplex) and three (triplex) cell populations:

    - R0K0 versus R6K6, R6K8, R10K8, R6K4 respectively.

    Leibovitz L-15 Medium


    Leibovitz media support the growth of hepatocytes and primary explants of embryonic and adult human tissue. Allowing physical pH control, the medium is buffered by phosphates and amino acids, so that sodium bicarbonate is not required. We offer 5 differently labelled products.

    • L-15 media allow improved growth and proliferation of a variety of cell lines and primary cells in an CO2 balanced environment including
    • - Primary explants of adult human and embryonic tissue
    • - Hep-2, llc –mk2
    • - Nerve cells
    • - L-15 media have been utilized also for cultivation of viruses.
    • Show More
    Order Number
    Media Description

    Packaging, Bottle

    L15-23-LM023
    Ready-to-use SILAC Leibovitz L15 Media containing 13C labelled arginine and lysine amino acids (R6K6)

    500 ml

    L15-24-LM024
    Control SILAC Leibovitz L15 Media containing unlabelled arginine and lysine amino acids (R0K0)

    500 ml

    L15-25-LM025
    Ready-to-use Leibovitz L15 Media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

    500 ml

    L15-26-LM026
    Normally used in triple labeling experiments.
    Ready-to-use SILAC Leibovitz L15 Media containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

    500 ml

    L15-27-LM027
    Normally used in triple labeling experiments.
    Ready-to-use SILAC Leibovitz L15 Media containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

    500 ml

    Q

    Notes: You can also find this product as Leibovitz's l-15 media, Leibovitz l-15, l-15 media, l-15 media ph, l-15 medium vs dmem, l-15 growth medium, Leibovitz l-15 media or l15 media, l15 medium or as l15 Leibovitz medium, l15 cell culture medium, or l15 media co2.



    SILAC IMDM – rapid Proliferation and Biomanufacturing.Enhance your high density cell cultures.

    Iscove's Modified Dulbecco's Medium (IMDM)


    IMDM (Iscove's modified Dulbecco's medium) is a totally serum-free synthetic medium and contains supplements comprising albumin, transferrin, lecithin and selenium as well as, amino acids, sodium selenite, sodium pyruvate, vitamins and inorganic salts, ferric nitrate and HEPES as buffer component.

    IMDM media, also known under the term Dulbecco’s modified eagle medium, can be used with serum or with reduced serum, expanding is use to a wide spectrum of mammalian cell types.

    IMDM is optimized for fast proliferation and for high-density cell cultures in a 5% CO2 environment. The medium supports growth of T and B lymphocytes and a variety of hybrid cells, also at reduced serum conditions. IMDM can be also used in biomanufacturing.


    SILAC - Stable Isotope Labeling with Amino Acids in Cell Culture – IMDM Dulbecco Modified is your choice for Mass Spectroscopy MS based quantitative proteomics.

    We offer 5 SILAC IMDM media with different label combinations for L-arginine and L-lysine, 13C, 15N and D2 (R6K4, R6K6, R6K8 and R10K8 respectively as well as control medium R0K0.) Each product is bottled at a volume of 500ml.

    IMDM media are used in certain cell and tissue cultures for growing cells and for biomanufacturing. Key application areas are
    - Protein identification
    - Biomarker Discovery
    - Proteome Analysis
    Order Number
    Media Description

    Packaging, Bottle

    IMDM-28-LM028
    Ready-to-use SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing 13C labelled arginine and lysine amino acids (R6K6)

    500 ml

    IMDM-29-LM029
    Control SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing unlabelled arginine and lysine amino acids (R0K0)

    500 ml

    IMDM-30-LM030
    Ready-to-use SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

    500 ml

    IMDM-31-LM031
    Normally used in triple labeling experiments.
    Ready-to-use SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

    500 ml

    IMDM-32-LM032
    Normally used in triple labeling experiments.
    Ready-to-use SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

    500 ml



    Remarks: You can also find this products as Dulbecco modified, IMDM vs DMEM, IMDM medium.



    Williams’ Medium E – SILAC enabled.Optimized for Long Term Hepatocyte Studies.

    WME - Williams Medium E - Williams Media

    William’s Medium E (WME) is an optimized medium for the long-term growth of adult liver epithelial cells. The formulation for Williams’ media E comprises special metal components such as iron, manganese and iron, the antioxidant glutathione as well as non-essential amino acids and lipid methyl linoleate.
    SILAC - Stable Isotope Labeling with Amino Acids in Cell Culture – Williams’ Medium E - is your choice for Mass Spectroscopy MS based quantitative proteomics.

    WME media are used in certain cell and tissue cultures for growing cells and for biomanufacturing. Key application areas are
    - Protein identification
    - Biomarker Discovery
    - Proteome Analysis

    Williams E medium has been utilized in a variety of studies:

    Primary hepatocyte isolation.
    Spheroid cultures.
    Study of cell cycle specificity of chemical mutagenesis.
    Study of apoptotic signaling pathway in metabolic disturbances occurring in liver tissue after cryopreservation mutagenesis.

    Literature:
    Study of cell cycle specificity of chemical mutagenesis. Proc. Natl. Acad. Soc.. USA, Vol. 77, No. 12, pp. 7377-7379, December 1980.
    Analysis of autophagy in isolated hepatocytes Autophagy in Disease and Clinical Applications, Part 3, edited by Daniel Klionsky.

    We offer five SILAC IMDM media with different label combinations for L-arginine and L-lysine, 13C, 15N and D2 (R6K4, R6K6, R6K8 and R10K8 respectively as well as control medium R0K0.) Each product is bottled at a volume of 500ml.

    Order Number
    Media Description

    Packaging, Bottle

    WME-33-LM033
    Ready-to-use SILAC Williams Medium E containing 13C labelled arginine and lysine amino acids (R6K6)

    500 ml

    WME-34-LM034
    Control SILAC Williams Medium E containing unlabelled arginine and lysine amino acids (R0K0)

    500 ml

    WME-35-LM035
    Ready-to-use SILAC Williams Medium E containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

    500 ml

    WME-36-LM036
    Normally used in triple labeling experiments.
    Ready-to-use SILAC Williams Medium E containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

    500 ml

    WME-37-LM037
    Normally used in triple labeling experiments.
    Ready-to-use SILAC Williams Medium E containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

    500 ml

    You can also find this product as Williams Medium, Williams Medium E, Williams Media E and Williams Cell Culture.



    Dulbecco’s F12 - DMEM Dulbecco – DMEM F12 - Reproducible Growth – Less Serum – Ready-to-go

    What is Dulbecco’s Modified Eagle Medium / Ham’s Nurient Mixture F12?
    The DMEM F12 formulation is the 1 to 1 blended richness of Ham’s F12 with the enhanced concentration of component from DMEM, Dulbecco’s Modified Eagle’s Medium. The DMEM F12 allows the serum free, at least the reduced serum culture of a variety of mammalian cells.

    What is the Usage of DMEM/F12 Media?
    The nutrient F12 composition is optimized for use as a basal medium for supporting superior growth, consistency and reliability. It can be applied reportedly for the growth of MDCK, glial cells, fibroblasts, human endothelial cell, rat fibroblasts and other. The F122 Medium supports clonal density cultures. F12 media are useful for enhancing the effect from growth factors and hormones on target tissues.

    Are there any specific Formulation Attributes?
    DMEM F-12 contains no proteins or growth factors. The medium contains HEPES as buffer component as a make-up for buffering capacity in the serum-free environment.

    What are the benefits of DMEM Dulbecco?
    • Overall better experimental results as a result of lower experimental variances.
    • DMEM F12 replaces conventional sera or allows reduced usage.
    • Improved experimental consistency due to elimination of serum changes.
    • Time and cost savings from shortfall of serum testing and reduced serum usage
    • Show More


    SILAC DMEM F12

    SILAC Stable isotope labeling by amino acids in cell culture is a well known technique for detection of differences in protein abundance among samples using non-radioactive isotopic labeling and mass spectrometry or NMR Nuclear Magnetic Resonance.

    SILAC is a robust and a superior reproducible method in quantitative proteomics with remarkable advantages:
    - Applicable to a wide range of cell types, supporting multiple culture conditions.
    - Robust experimental results due to labelling prior to sample preparation.
    - Ultra high labelling efficiency and high dynamic range in detection.

    SILAC Dulbecco’s F12 media incorporate L-Arginine and L-Lysine isotopes for quantitative proteomics. We offer following isotopes for the comparison of cell populations:
    - R0K0 versus R6K6, R6K8, R10K8, R6K4 respectively.

    Order Number
    Media Description

    Packaging, Bottle

    DMEF-38-LM038
    Control SILAC DMEM F12 media containing unlabeled arginine and lysine amino acids (R0K0)

    500 ml

    DMEF-39-LM039
    Ready-to-use SILAC DMEM F12 media containing 13C labelled arginine and lysine amino acids (R6K6)

    500 ml

    DMEF-40-LM040
    Ready-to-use SILAC DMEM F12 media containing 13C and 15N labelled arginine, and 13C and 15N labelled lysine (R10K8). Normally used in triple labelling experiments

    500 ml

    DMEF-41-LM041
    Ready-to-use SILAC DMEM F12 media containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

    500 ml

    DMEF-42-LM042
    Ready-to-use SILAC DMEM F12 media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

    500 ml




    McCoy's 5A (modified) Medium.Universal as Medium – Universal for Growth.

    McCoy's 5A (modified)


    McCoys 5A (modified) Medium, also named McCoys 5a medium, is a universal medium. In total, due to its universal applications, 5A media are generally used for growing primary cultures. The medium is beneficial for the propagation of leukocytes, a wide range of human and rat normal or transformed cells types and for biopsy tissues.

    Typical target cells in the field of primary mammalian and established cell types are for example 293T, A431, A549, BMMN, COS7, HCT116, HeLa, HepG2, HT-29, K562, NIH 3T3, or U2OS.

    McCoys 5A medium is utilized for explants from tissues derived from biopsies, such as adrenal glands, gingiva, intestine, kidney, liver, lung, normal bone marrow, rat embryos, skin or spleen.

    As the Mc Coy‘s medium is used as universal medium, it contains all required amino acids and vitamins. It can be used in ambient environment, e.g. outside of CO2 incubators, due to enrichment with Hank’s salts HBSS.

    SILAC McCoy’s 5A Medium

    SILAC Stable isotope labeling by amino acids in cell culture is a well known technique for detection of differences in protein abundance among samples using non-radioactive isotopic labeling and mass spectrometry or NMR Nuclear Magnetic Resonance.

    SILAC is a robust and a superior reproducible method in quantitative proteomics with remarkable advantages:
    - Applicable to a wide range of cell types, supporting multiple culture conditions
    - Robust experimental results due to labeling prior to sample preparation
    - Ultra high labeling efficiency and high dynamic range in detection

    SILAC McCoys 5A Media incorporate L-Arginine and L-Lysine isotopes for quantitative proteomics. We offer following isotopes for the comparison of cell populations:
    - R0K0 versus R6K6, R6K8, R10K8, R6K4 respectively.

    Order Number
    Media Description

    Packaging, Bottle

    MCM-48-MCM048
    Ready-to-use SILAC McCoy`s 5A Medium (Modified) containing 13C labelled arginine and lysine amino acids (R6K6)

    500 ml

    MCM-49-MCM049
    Control SILAC McCoy`s 5A Medium (Modified) containing unlabeled arginine and lysine amino acids (R0K0)

    500 ml

    MCM-50-MCM050
    Ready-to-use SILAC McCoy’s 5A Medium (Modified) containing 13C labeled arginine and lysine amino acids (R6K8)

    500 ml

    MCM-51-MCM051
    Ready-to-use SILAC McCoy’s 5A Medium (Modified) containing 13C labeled arginine and lysine amino acids (R10K8)

    500 ml

    MCM-52-MCM052
    Ready-to-use SILAC McCoy’s 5A Medium (Modified) containing 13C labeled arginine and lysine amino acids (R6K4)

    500 ml




    Schneider’s Insect Medium - DrosophilaHigh Growth and Efficient Protein Expression

    Schneider Insect Medium / Schneider Drosophila Medium


    Insect cells are capable to express large amounts of proteins with complex post-translational combinations. That is why they are a preferred choice for the production of biologicals. Their advanced growth flexibility compared to mammalian or bacterial cell lines can be beneficially used in the BEVS Baculovirus expression vector system.

    Schneider’s Insect Medium is a preferred medium in genetic, molecular biology and cell research. Also named as Schneider’s Drosophila Medium, it is important for the study of heterologous protein expression. It generates rapid growth of cell derived from Drosophila and other species of the order diptera.

    SILAC Schneider’s Insect Medium.

    SILAC Stable isotope labeling by amino acids in cell culture is a well known technique for detection of differences in protein abundance among samples using non-radioactive isotopic labeling and mass spectrometry or NMR Nuclear Magnetic Resonance.

    SILAC is a robust and a superior reproducible method in quantitative proteomics with remarkable advantages:
    - Applicable to a wide range of cell types, supporting multiple culture conditions
    - Robust experimental results due to labeling prior to sample preparation
    - Ultra high labeling efficiency and high dynamic range in detection

    SILAC Schneider’s Insect Medium incorporates L-Arginine and L-Lysine isotopes for quantitative proteomics. We offer following isotopes for the comparison of cell populations:
    - R0K0 versus R6K6, R6K8, R10K8, R6K4 respectively.

    Order Number
    Media Description

    Packaging, Bottle

    SCM-43-MI043
    Ready-to-use SILAC Schneider insect / drosophila medium containing 13C labelled arginine and lysine amino acids (R6K6)

    500 ml

    SCM-44-MI044
    Control SILAC Schneider insect / drosophila medium containing unlabelled arginine and lysine amino acids (R0K0)

    500 ml

    SCM-45-MI045
    Ready-to-use SILAC Schneider insect / drosophila medium containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

    500 ml

    SCM-46-MI046
    Ready-to-use SILAC Schneider insect / drosophila medium containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

    500 ml

    SCM-47-MI047
    Ready-to-use SILAC Schneider insect drosphila medium containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

    500 ml

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