Cryopreserved Cynomolgus Hepatocytes

The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins under physiological and pathophysiological conditions.

Hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. In this biotransformation process transporter proteins (influx and efflux transporter), phase I reactions (cytochrome P450 proteins), phase II reactions (mainly glucuronidation and sulfatation) play a central role.

Cryopreserved primary hepatocytes are ideally suited for in vitro metabolism and toxicity/detoxification studies before preclinical or clinical tests.

Monkey hepatocytes are isolated from livers obtained from male or female Cynomolgus and are cryopreserved directly after isolation. Donor demographics stating vaccination status and health status of the animals are available for each animal. Cryopreserved Cynomolgus hepatocytes are used in suspension assays and plated in 2D and 3D cell cultures.

PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as a member of the laboratory network responsible for the method validation.

Our colleagues are professionals and undergo continuous training in the preparation, isolation, and cryopreservation of hepatocytes in general, including related co-products, methods, and tools. We are a GLP certified laboratory since 2006.

Primacyt offers certified pools of cryopreserved Cynomolgus hepatocytes and hepatocytes from single donors. Pooled hepatocytes are obtained from three individual animal liver perfusions performed in parallel. After quality control, a pool consisting of equal numbers of viable liver cells from each donor is cryopreserved. Also, hepatocytes from individual donors are cryopreserved for reference purposes.

Each pool and the individual single donors are comprehensively tested for viability, plate-ability, Cytochrome P450/Phase I and Phase II activities to ensure that all donors are represented equally within the pool.

Cryopreserved hepatocytes from individual donors are also tested for drug transporter activities.

View the three page factsheet for Cynomolgus Monkey Hepatocytes

This info refers to the product data below:
• Inventory: Number of vials available as of the date of the last update. Availability of vials may have changed since that date by interim orders.
• Viability: Denotes post-thaw results using the thawing protocol for hepatocytes.
• To print lot information, please select the print icon at the end of the lists below. For research use only.
• Not intended for human or animal diagnostic or therapeutic use.