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Fresh Rat Hepatocytes from PRIMACYT


Primacyt provides primary rat hepatocytes and non-parenchymal liver cells for 2D and 3D cell cultures. They mimic hepatocellular functions.

Primary hepatocytes are
perfect models for in vitro metabolism and toxicity studies. They are essential before clinical in vivo assessments and for bioaccumulation and clearance studies,

The liver fulfills many vital processes in the body for energy metabolisms, such as glycolysis, gluconeogenesis, lipid, and amino acid metabolisms.

Under physiological and pathophysiological conditions, the liver is responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins, ammonia detoxification (ureagenesis), and drug metabolism.
Parenchymal hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. As part of this biotransformation process, influx and efflux transporter proteins, together with phase I reactions (cytochrome P450 proteins), and phase II reactions, primarily glucuronidation and sulfation, play a central role.

NPCs play a pivotal role during inflammatory response and fibrosis, interacting with hepatocytes and complementing liver-specific functions. Non-parenchymal liver cells, NPCs, include Kupffer cells, endothelial cells, and fat-storing cells (Ito cells, hepatic stellate cells).

Primacyt provides rat mouse hepatocytes and non-parenchymal liver cells for 2D and 3D cell cultures. They mimic hepatocellular functions.

Primary hepatocytes are
perfect models for in vitro metabolism and toxicity studies. They are essential before clinical in vivo assessments and for bioaccumulation and clearance studies,

The liver fulfills many vital processes in the body for energy metabolisms, such as glycolysis, gluconeogenesis, lipid, and amino acid metabolisms.

Under physiological and pathophysiological conditions, the liver is responsible for the maintenance of the blood sugar level and the synthesis of plasma proteins, ammonia detoxification (ureagenesis), and drug metabolism.


Parenchymal hepatocytes are the most prominent cells within the liver. Hepatocytes eliminate toxic substances from the blood. As part of this biotransformation process, influx and efflux transporter proteins, together with phase I reactions (cytochrome P450 proteins), and phase II reactions, primarily glucuronidation and sulfation, play a central role.

NPCs play a pivotal role during inflammatory response and fibrosis, interacting with hepatocytes and complementing liver-specific functions. Non-parenchymal liver cells, NPCs, include Kupffer cells, endothelial cells, and fat-storing cells (Ito cells, hepatic stellate cells).



Our fresh Rat hepatocytes are of highest quality. The level of quality insurance is identical with human hepatocytes. Typically we offer highest viability and functionality levels. In combination with appropriate cell morphology, our cell technology is one instrument for adequate in vitro - in vivo correlations for your purposes.
Characterization methods of fresh animal hepatocytes include the monitoring of liver specific functions, activities of drug transporters and the induction of cytochrome P450 activities. We also monitor and document cell morphology, attachment efficiency, monolayer confluency for platable cells and the overall appearance over time in culture.
Our fresh Rat hepatocytes are of highest quality. The level of quality insurance is identical with human hepatocytes. Typically we offer highest viability and functionality levels. In combination with appropriate cell morphology, our cell technology is one instrument for adequate in vitro - in vivo correlations for your purposes.

Characterization methods of fresh animal hepatocytes include the monitoring of liver specific functions, activities of drug transporters and the induction of cytochrome P450 activities. We also monitor and document cell morphology, attachment efficiency, monolayer confluency for platable cells and the overall appearance over time in culture.

Rat hepatocytes are available in suspension and in multi-well plates (6-, 12-, 24- and 96-well plate).We offer liver cells from Wistar:Han rats as our standard products. Fresh liver cells from other strains, e.g. Sprague Dawley or others can be isolated on request.

  • Products in SuspensionSuspensions will be sent on crushed ice on the day of isolation and should be used on the day of receipt. Instructions for the recovery of cells are included with each shipment. Although hepatocytes in suspension are normally plateable within 20-24 hours after isolation, we do not guarantee the platability of these cells, and recommend to order plated hepatocytes if this is the intended use.
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  • Plated ProductsPlated products will be sent at ambient temperature on day 1 after isolation and stored at 37°C with 95% humidity and 5% CO2 atmosphere. Included with each plated order are the following documents: Animal Demographics, datasheet (with microscopic images of cells before shipping), manual for the culture of primary hepatocytes.
  • Show More
  • Products in SuspensionSuspensions will be sent on crushed ice on the day of isolation and should be used on the day of receipt. Instructions for the recovery of cells are included with each shipment. Although hepatocytes in suspension are normally plateable within 20-24 hours after isolation, we do not guarantee the platability of these cells, and recommend to order plated hepatocytes if this is the intended use.
  • Show More

  • Plated ProductsPlated products will be sent at ambient temperature on day 1 after isolation and stored at 37°C with 95% humidity and 5% CO2 atmosphere. Included with each plated order are the following documents: Animal Demographics, datasheet (with microscopic images of cells before shipping), manual for the culture of primary hepatocytes.
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Fresh Rat Hepatocytes (Wistar:Han) in Suspension.

Fresh Sprague Dawley Hepatocytes on Request.


Minimum Order 30 Million Cells.
Product No.
Product
Specification
Packaging
EUR per 1 Mio Cells - Wistar Han

RHS-01

In suspension

> 30 Mio viable cells

Vial

38.00

RHS-01

In suspension

> 50 Mio viable cells

Vial

34.00

Quantity:





-190104

Fresh Rat Hepatocytes (Wistar:Han) Plated.

Fresh Sprague Dawley Hepatocytes on Request.


It is recommended to culture Rat hepatocytes on collagen-coated culture plates in HHMM (Human Hepatocyte Maintenance Medium), a serum-free culture medium containing Hepatocyte Growth and Epidermal Growth Factor. Culture in HGM (Hepatocyte Growth Medium) will also lead to favorable results.
Cells in plates require light-protected storage at 37 ℃ and 5 % CO2..

Minimum Order: 2 Plates.
Product No.
Product
Specification
Packaging
EUR per 1 Mio Cells - Wistar Han

RHP-06

6 well plate
collagen coated

1.2 Mio cells per well

1-2 plates

Please ask

RHP-06

6 well plate
collagen coated

1.2 Mio cells per well

3-5 plates

340.00

RHP-06

6 well plate
collagen coated

1.2 Mio cells per well

6-10 plates

315.00

RHP-06

6 well plate
collagen coated

1.2 Mio cells per well

> 10 plates

295.00

Quantity:




Product No.
Product
Specification
Packaging
EUR per Plate - Wistar Han

RHP-12

12 well plate
collagen coated

600.000
cells per well

1-2 plates

Please ask

RHP-12

12 well plate
collagen coated

600.000
cells per well

3-5 plates

340.00

RHP-12

12 well plate
collagen coated

600.000
cells per well

6-10 plates

315.00

RHP-12

12 well plate
collagen coated

600.000
cells per well

> 10 plates

295.00

Quantity:





Product No.
Product
Specification
Packaging
EUR per Plate - Wistar Han

RHP-24

24 well plate
collagen coated

300.000
cells per well

1-2 plates

Please ask

RHP-24

24 well plate
collagen coated

300.000
cells per well

3-5 plates

340.00

RHP-24

24 well plate
collagen coated

300.000
cells per well

6-10 plates

315.00

RHP-24

24 well plate
collagen coated

300.000
cells per well

> 10 plates

295.00

Quantity:



Product No.
Product
Specification
Packaging
EUR per Plate - Wistar Han

RHP-96

96 well plate
collagen coated

50.000
cells per well

1-2 plates

Please ask

RHP-96

96 well plate
collagen coated

50.000
cells per well

3-5 plates

340.00

RHP-96

96 well plate
collagen coated

50.000
cells per well

6-10 plates

315.00

RHP-96

96 well plate
collagen coated

50.000
cells per well

> 10 plates

295.00

Quantity:




Biohazard warning: Tissue fractions such as hepatocytes should be considered as potentially biohazardous, and should be treated as biohazards in the laboratory.
Only for research purposes. Not for use in human diagnostics or therapeutics.