PRIMACYT Subcellular Fractions - Choose from our large collection, containg a wide variety of species. High quality - Single and Pooled Donors - Characterized and validated.
Primacyt has introduced a portfolio of liver subcellular fractions, available as an S9 fraction, liver cytosol, and liver microsomes. Products are from single donors, male or female. Microsomes and S9 fractions are characterized by CYP metabolism / SDS PAGE, Cytosol by SDS PAGE. For all fractions, the Protein content in mg/ml is availableSubcellular fractions are suitable for a variety of experiments including Metabolic Stability, Cytochrome P450 Phenotyping, Metabolite Characterization, Cytochrome P450 Inhibition Studies, Metabolic Stabilit, Cytochrome P450 Phenotyping, and Metabolite Characterization.
Precinical studies require the understandíng the potenial interaction between novel candidates and other drugs including information about the underlying pathways. The liver is predominant for the metabolism for endogenous compounds and xenobiotics. Enzymes contained in the Cytochrome P450 superfamily of the liver are used predominantly in invitro drug biotransformation studies.
PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation. Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
PRIMACYT provides subcellular liver fractions from human and a variety of animals for use in drug development in human and animal health. Also, liver cells and subcellular liver fractions are provided for environmental toxicology studies of herbicide and pesticide metabolism and bioaccumulation studies in birds and fish, like rainbow trout (Oncorhynchus mykiss) or Atlantic salmon (Salmo salar). The Liver microsome, S9 fractions, and cytosolic extracts are characterized by SDS-PAGE analysis and the determination of Cytochrome P450 activities. In subcellular fractions derived from human donors, we monitor the specific activities of cytochrome P450 and UDP-glucuronosyl-transferases (UGT).
Animal Tissue Performance and Regulations. The acquisition of animal tissue is performed under permits 3902-046-2017 and DE 13 076 0001 21 from the Landkreis Ludwigslust-Parchim, Department of Veterinary- and Foodstuff Control, following EU Regulation No. 1029/2009, Art. 17 and 23 and permit 7221.3-2-004/17 from the Ministry for Agriculture, Food Safety and Fishery of the State Mecklenburg-Pomerania in accordance with EU Regulation 2010/63/EU, and with several additional regulations for the protection of animals (e.g., TierSchG dated July 18, 2016, and TierSchVerV dated August 31, 2015) used for experimentation and other scientific purposes.
Subcellular fractions derived from liver contain a rich variety of metabolic enzymes for assessing the in vitro metabolism of drug candidates and xenobiotics. They are used to evaluate active pharmaceutical ingredients in human and animal health. Other applications include the environmental toxicological assessment of agrochemicals and the evaluation of cosmetics and food additives.