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Your Tool for Drug Discovery and Drug Development.

PRIMACYT provides subcellular liver fractions from human and a variety of animals for use in drug development in human and animal health. Also, liver cells and subcellular liver fractions are provided for environmental toxicology studies of herbicide and pesticide metabolism and bioaccumulation studies in birds and fish, like rainbow trout (Oncorhynchus mykiss) or Atlantic salmon (Salmo salar). S9 fractions and microsomes from species not listed above, e.g., turkey (Meleagris gallopavo), Mallard duck (Anas platyrhynchos) or Japanese quail (Coturnix japonica) can be made available upon request.

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    Common Carp - Overview
    Animals like Common Carp have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, herbicides and crop protection products. The increasing economic importance of food production in aquacultures requires the analysis of plant-based ingredients in aquafeeds concerning food-borne mycotoxins.

    Further Application Fields

    Subcellular fractions are suitable for a variety of experiments including

    - Cytochrome P450 Inhibition Studies
    - Metabolite Characterization
    - Metabolic Stability

    S9 subcellular fractions derived from liver contain microsomes and cytosol and express a rich variety of metabolic enzymes for assessing the in vitro metabolism of drug candidates and xenobiotics (Phase I Cytochrome P450 enzymes, Flavin mono-oxygenases (FMO), UGT-glucuronyltransferases (UGT), Carboxylesterases and soluble Phase II proteins like N-acetyltransferase, Sulfotransferases (SULT), and Glutathione-S-Transferases (GST)). They are used to evaluate active pharmaceutical ingredients in human and animal health. Other applications include the environmental and toxicological assessment of agrochemicals and the evaluation of cosmetics and food additives.


    Our Competencies
    Your Primacyt Experts in Biology pertaining to the Liver

    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
    Common Carp - a new in-vitro System

    03 Carp Liver subcellular fractions



    Developing regulatory activities (e.g., REACh) require bioaccumulation to be assessed for thousands of chemicals. On top of this, there is increasing pressure to reduce, refine and replace animal tests. This leads to an urgent need for the evaluation of in vitro systems to provide significant data for the estimation of bioaccumulation of chemicals of all kind in human and animals. We at Primacyt provide these in vitro systems to researchers worldwide.

    Carp liver subcellular fractions have been used in aquatic toxicology to analyze metabolism, bioaccumulation and toxic effects of, e.g., pesticides, pharmaceuticals, flame retardants, surfactants, insecticides, and acaricides.



    Literature
    Chen M, Guo T, He K, Zhu L, Jin H, Wang Q, Liu M, and Yang L: Biotransformation and bio-concentration of 6:2 and 8:2 polyfluoroalkyl phosphate diesters in common carp (Cyprinus carpio): Underestimated ecological risks. Sci. Total Environ., 2019, 656:201-208

    Bearr JS, Mitchelmore CL, Roberts SC, Stapleton HM: Species specific differences in the in vitro metabolism of the flame retardant mixture, Firemaster BZ-54. Aquatic Toxicology, 2012, doi:10.1016/j.aquatox.2012.06.006

    Shen M, Cheng J, Wu R, Zhang S, Mao L, Gao S: Metabolism of polybrominated diphenyl ethers and tetrabromobisphenol A by fish liver subcellular fractions in vitro. Aquatic Toxicology, 2012, 114-115: 73-79

    Cowan-Ellsberry CE, Dyer SD, Erhardt S, Bernhard MJ; Roe AL, Dowty ME, Weisbrod AV: Approach for extrapolating in vitro metabolism data to refine bio-concentration factor estimates. Chemosphere 2008, 70:1804-18017



    Our Offering
    Primacyt now offers cryopreserved carp liver S9 fractions from sexually immature animals. Thereby, we add a new in-vitro model system obtained from non-predatory freshwater fish to our already existing portfolio of liver-derived cellular and sub-cellular in vitro systems of predator fish from fresh and salt-water.


    Additional Product Info
    This info refers to the product data below:
    • Inventory: Number of vials available as of the date of the last update. Availability of vials may have changed since that date by interim orders.
    • To print lot information, please select the print icon at the end of the lists below.
    • For research use only. Not intended for human or animal diagnostic or therapeutic use.
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    Download Area MSDS
    Please click on the buttons below to download MSDS data and the Plating and Thawing Manual.





    Common Carp - subcellular fraction, Cryopreserved, Pool – Lot and Order Information

    Product Codes CCl-S9-1P2

    -20190117

    Product No.
    Product
    Lot No.
    Gender
    Specification
    Characterisation
    Protein content (mg/ml)
    Unit size (ml/vial)
    Available vials
    Euro per vial
    CCL-S9-1P2
    Common carp liver S9 fraction
    180704
    male
    pool of 2
    Phase I and Phase II, SDS page

    20

    0,5

    34

    68,00

    Q

    Product Codes CCL-S9-1P2

    Quantity:


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    Download Lot Information



    GSN-185755-I