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Your Tool for Drug Discovery and Drug Development.


PRIMACYT provides subcellular liver fractions from human and a variety of animals for use in drug development in human and animal health. Also, liver cells and subcellular liver fractions are provided for environmental toxicology studies of herbicide and pesticide metabolism and bioaccumulation studies in birds and fish, like rainbow trout (Oncorhynchus mykiss) or Atlantic salmon (Salmo salar). S9 fractions and microsomes from species not listed above, e.g., turkey (Meleagris gallopavo), Mallard duck (Anas platyrhynchos) or Japanese quail (Coturnix japonica) can be made available upon request.

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    Overview
    Animals like Atlantic Salmon have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavoring substances, herbicides and crop protection products. Apart from Zebrafish, Rainbow trout is the best-studied fish species.

    The OECD Test Guideline 319B Determination of in vitro intrinsic clearance using rainbow trout liver subcellular fraction (RT-S9) was released on June 25, 2018. Primacyt now offers cryopreserved pools of rainbow trout S9 fractions prepared and characterized in accordance with this guideline.

    Further Application Fields

    Subcellular fractions are suitable for a variety of experiments including

    - Cytochrome P450 Inhibition Studies
    - Metabolite Characterization
    - Metabolic Stability
    - Cytochrome P450 Phenotyping

    Subcellular fractions derived from liver contain a rich variety of metabolic enzymes for assessing the in vitro metabolism of drug candidates and xenobiotics. They are used to evaluate active pharmaceutical ingredients in human and animal health. Other applications include the environmental toxicological assessment of agrochemicals and the evaluation of cosmetics and food additives.

    Our Competencies
    Your Primacyt Experts in Biology pertaining to the Liver

    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
    Rainbow Trout - a new in-vitro System
    More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have introduced trout hepatocytes as a reliable in-vitro system for bioaccumulation and clearances studies.

    Measurements of biotransformation rates in primary fish hepatocytes can be scaled up to the whole animal and thereby can provide data used in computational models to predict chemical bio-concentration factors in animals (K.A. Faye et al. 2014, I. Bischof et al. 2016).

    These and other supporting research results lead to the implementation of OECD Test Guidelines 319A and 319B on the determination of intrinsic clearance using cryopreserved rainbow trout hepatocytes and liver S9 subcellular fractions.

    Access the webpage for this guideline and download the document:


    Literature
    Literature
    Intra- and interlaboratory reliability of a cryopreserved trout hepatocyte assay for the prediction of chemical bioaccumulation potential.

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    Hepatocytes as in vitro test system to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species.

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    Reliability of In Vitro Methods used to Measure Intrinsic Clearance of Hydrophobic Organic Chemicals by Rainbow Trout: Results of an International Ring Trial.

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    Our Offering
    In accordance with OECD Guideline 319B Determination of in vitro intrinsic clearance using rainbow trout liver subcellular fraction (RT-S9).

    Primacyt now offers cryopreserved pools liver S9 fractions obtained from rainbow trout kept under controlled conditions at Fraunhofer Institute for Marine Biotechnology and Cell Technology for at least two weeks in continuous flow water.

    Additional Product Info
    This info refers to the product data below:
    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the thawing protocol for fish hepatocytes.
    • To print lot information, please select the print icon at the end of the lists below.
    • For research use only. Not intended for human or animal diagnostic or therapeutic use.
    Contact us now
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    Download Area MSDS
    Please click on the buttons below to download MSDS data and the Plating and Thawing Manual.





    Rainbow subcellular fraction, Cryopreserved, Pool of sexually immature animals kept under controlled husbandry conditions – Lot and Order Information

    Product Codes RTL-S9-3P6

    -20190117

    Product No.
    Product
    Lot No.
    Gender
    Specification
    Characterisation
    Protein content (mg/ml)
    Unit size (ml/vial)
    Available vials
    Euro per vial
    RTL-S9-3P6
    Rainbow trout liver S9 fraction
    Rainbow trout 180216
    mixed
    pool of 6 (3m, 3f)
    Phase I and Phase II metabolism, SDS PAGE

    20

    0,5

    75

    77,00

    Q

    Product Codes RTL-S9-3P6

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.
    Download Lot Information





    Rainbow subcellular fraction, Cryopreserved, Pool of animals from non-controlled husbandry conditions – Lot and Order Information

    -20190117

    Product No.
    Product
    Lot No.
    Gender
    Specification
    Characterisation
    Protein content (mg/ml)
    Unit size (ml/vial)
    Available vials
    Euro per vial
    FL-S9-2P10
    Rainbow trout liver S9 fraction
    Rainbow trout 170613
    female
    pool of 10
    Phase I metabolism, SDS PAGE

    20

    1,0

    74

    70,40

    FL-MIC-2P10
    Rainbow trout liver S9 fraction
    Rainbow trout 170613
    female
    pool of 10
    Phase I metabolism, SDS PAGE

    20

    1,0

    64

    105,60

    Q

    Product Codes FL-S9-2P10 / FL-MIC-2P10

    Quantity:


    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.
    Download Lot Information



    GSN-185755-I