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PRIMACYT is one of the pioneers in the development of advanced in vitro model systems. We are a leading Company in the field of research of human and animal hepatocytes. We offer fresh and cryopreserved animal hepatocytes in suspension and plated.

Cryopreserved Rainbow Trout (Oncorhynchus mykiss) Hepatocytes from PRIMACYT.

Pooled Fish Hepatocytes - a new in-vitro model metabolic System.


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    Overview
    Animals like Rainbow trout (Oncorhynchus mykiss) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavoring substances, herbicides and crop protection products. Apart from Zebrafish, Rainbow trout is the best-studied fish species.
    Our Competencies
    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.
    Rainbow Trout - a new in-vitro System
    More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have introduced trout hepatocytes as a reliable in-vitro system for bioaccumulation and clearances studies. Measurements of biotransformation rates in primary fish hepatocytes can be scaled up to the whole animal and thereby can provide data used in computational models to predict chemical bio-concentration factors in animals (K.A. Faye et al. 2014, I. Bischof et al. 2016). These and other supporting research results lead to the draft of an OECD Test Guideline on the determination of intrinsic clearance using cryopreserved rainbow trout hepatocytes and liver S9 subcellular fractions.
    Literature
    Literature:
    K.A. Faye, R.T. Mingoia, I. Göritz, D.L. Nabb, A.D. Hoffman, B.D. Ferrel, H.M. Peterson, J.W. Nichols, H. Segner, and X. Han: Intra- and Interlaboratory Reliability of a Cryopreserved Trout Hepatocyte Assay for the Prediction of Chemical Bioaccumulation Potential. Environmental Science & Technology 2014, 48, 8170-8178

    I. Bischof, J. Köster, H. Segner, and C. Schlechtriem: Hepatocytes as in vitro test system to investigate to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species. Comparative Biochemistry and Physiology, Part C, 2016, 187, 62-73
    Our Offering
    Primacyt now offers cryopreserved pools of primary hepatocytes from rainbow trout kept under controlled conditions at Fraunhofer Institute for Marine Biotechnology and Cell Technology for at least two weeks in continuous flow water. Each lot is comprehensively tested for high viability and Cytochrome P450/Phase I and Phase II activities.
    Download Area
    Please click on the buttons below to download MSDS data and the Plating and Thawing Manual.




    Additional Product Info
    This info refers to the product data below:
    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select the print icon at the end of the lists below.
    Contact us now
    Click on the color field below to ask fopr more information.

    Animals like Rainbow trout (Oncorhynchus mykiss) have been used in vivo by researchers for decades to assess bioaccumulation, metabolism, and environmental risk of chemicals, including drugs, xenobiotics, cosmetics, fragrances, flavoring substances, herbicides and crop protection products. Apart from Zebrafish, Rainbow trout is the best-studied fish species.

    More recently, researchers of the US Environmental Protection Agency (EPA), DuPont Haskell, the University of Bern and Fraunhofer IME have introduced trout hepatocytes as a reliable in-vitro system for bioaccumulation and clearances studies. Measurements of biotransformation rates in primary fish hepatocytes can be scaled up to the whole animal and thereby can provide data used in computational models to predict chemical bio-concentration factors in animals (K.A. Faye et al. 2014, I. Bischof et al. 2016). These and other supporting research results lead to the draft of an OECD Test Guideline on the determination of intrinsic clearance using cryopreserved rainbow trout hepatocytes and liver S9 subcellular fractions.

    Primacyt now offers cryopreserved pools of primary hepatocytes from rainbow trout kept under controlled conditions at Fraunhofer Institute for Marine Biotechnology and Cell Technology for at least two weeks in continuous flow water. Each lot is comprehensively tested for high viability and Cytochrome P450/Phase I and Phase II activities.

    Literature:
    K.A. Faye, R.T. Mingoia, I. Göritz, D.L. Nabb, A.D. Hoffman, B.D. Ferrel, H.M. Peterson, J.W. Nichols, H. Segner, and X. Han: Intra- and Interlaboratory Reliability of a Cryopreserved Trout Hepatocyte Assay for the Prediction of Chemical Bioaccumulation Potential. Environmental Science & Technology 2014, 48, 8170-8178

    I. Bischof, J. Köster, H. Segner, and C. Schlechtriem: Hepatocytes as in vitro test system to investigate to investigate metabolite patterns of pesticides in farmed rainbow trout and common carp: Comparison between in vivo and in vitro and across species. Comparative Biochemistry and Physiology, Part C, 2016, 187, 62-73


    PRIMACYT is approved by the European Commission as an EU Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM) and serves as member of the laboratory network responsible for the method validation.

    Our colleagues are professionals and undergo continuous training in the preparation, isolation and cryopreservation of hepatocytes in general, including related co-products, methods and tools. We are a GLP certified laboratory since 2006.

    Download MSDS

    Thawing and Plating Manual




    Cryopreserved Rainbow Trout (Oncorhynchus mykiss) Hepatocytes from PRIMACYT.

    • Inventory: Number of vials available as of date of last update. Availability of vials may have changed since that date by interim orders.
    • Viability: Denotes post thaw results using the standard thawing protocol.
    • To print lot information, please select print icon at the end of this list.

    Product Code RTHCS - Rainbow Trout Hepatocytes Cryopreserved for use in Suspension - Pools of sexually immature animals kept under controlled husbandry conditions – Lot and Order Information

    Click on the Lot-Info Hyperlink to see the detailed Lot Information.
    Product CodeLOTGenderInventoryViability %Total cells x106/vialViability %Recovery x106/vialTotal number of cells available x106Viability after shaking for 2h at 14°CPrice EUR/Vial
    RTHCSRTH180212-1 Pool2 m, 1 f449515893.515496320.00
    RTHCSRTH180212-2 Pool1 m, 2 f89610973.02493320.00
    RTHCSRTH180214-1 Pool3 f619710985.634196440.00
    RTHCSRTH180214-2 Pool2 m89710986.45194440.00
    RTHCSRTH180219 Pool6 f879710953.026197320.00
    RTHCSRTH180221 Pool2 m, 2 f759710985.843598440.00
    Q


    Product Code RTHCS
    Price per Vial is determined by the number of cells according to the table below:
    < 4 Mio - EUR 320,00
    more than 4 Mio - 440,00

    Quantity:

    You need fresh trout or other fish species hepatocytes? Please ask.

    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.
    .


    Download Lot Information





    Product Code FHCS - Cryopreserved trout hepatocytes for use in Suspension - Single donors, animals kept under non-controlled husbandry conditions – Lot and Order Information

    Click on the Lot-Info Hyperlink to see the detailed Lot Information.
    Product CodeLOTGenderInventoryViability %Total cells x106/vialViability %Recovery x106/vialTotal number of cells available x106Viability (%) after shaking for 1h at 37 deg CPrice (EUR)
    FHCSFH160727-4M89510.2989.072.090.6473.00
    FHCSFH160907-1M89410.3942.923.294.2236,50
    FHCSFH160907-2F109510.5944.04096.1354.75
    Q


    Product Code FHCS
    Price per Vial is determined by the number of cells according to the table below:
    2 - 3.9 Mio - EUR 236,50
    4 - 5.9 Mio - EUR 354,75
    6 - 9.9 Mio - EUR 473.00

    Quantity:

    You need fresh trout or other fish species hepatocytes? Please ask.

    Get in Dialog with PRIMACYT and request a Quote by preformatted Email.
    .



    Download Lot Information


    GSN-185755-I